FIGURE 2.

Ferroptosis inhibitors reversed 4HC-induced cytotoxicity (A–B) GL261 and 4T1 cell lines were treated with different concentrations of 4HC (0–160 μM) for 6, 12, 24 or 48 h (C–D) Cell morphology was photographed under an inverted microscope, and representative images from experiments with similar results are shown (scale bar = 500 μm) (E) GL261 cell line was preincubated with Fer1 (10 μM) or DFO (400 μM) for 1 h followed by 4HC (0–160 μM) for 24 h. The values of cell viability and cytotoxicity are presented as the means ± SD from more than three independent experiments.*p < 0.05, **p < 0.01, and ***p < 0.001 (one-way ANOVA analysis) for the indicated comparisons.