FIGURE 4.

Ferroptosis inhibitors reversed the 4HC-induced decrease of the mitochondrial membrane potential. (A–B) Images of JC-1 staining in 4HC-treated GL261 and 4T1 cells cultured in the presence or absence of Fer1 and DFO. Shown were representative images with similar results (scale bar = 500 μm). (C–D) Determination and flow cytometry analysis of the MMP in tumor cells treated under the indicated conditions. Values are presented as the means ± SD from more than three independent experiments. ns, not significant, *p < 0.05, **p < 0.01, and ***p < 0.001 (one-way ANOVA analysis) for the indicated comparisons.