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. 2022 Feb 16;14(3):e14901. doi: 10.15252/emmm.202114901

Figure EV3. The maternal OTULIN variant p.W167S more severely impairs hydrolysis of linear ubiquitin linkages than the paternal OTULIN variant p.M86I.

Figure EV3

  • A, B
    A549 OTULIN KO cells were transfected with different OTULIN constructs as indicated. After 24 h, cells were lysed and subjected to analysis by Western blot. One representative of three independent experiments is shown.
  • C
    OTULIN protein expression in B cells derived from control, patient, mother, and father is shown. Blots (left panel) are representative of five independent experiments analyzed by densitometry (right panel). Data are presented as mean ± SD (dots represent individual experiments); ***P = 0.0008, **P = 0.0069, ordinary one‐way ANOVA, Dunnett’s multiple comparisons test.
  • D
    Relative mRNA expression of OTULIN in B cells derived from control, patient, mother, and father determined with two different primer pairs is depicted. Data are presented as mean ± SD of four independent experiments; dots represent individual experiments performed in three technical replicates; ns, non‐significant, ordinary one‐way ANOVA, Dunnett’s multiple comparisons test.