Figure 3. Enteric hyperoxaluria in silico simulation (ISS) schematic.

1. Oxalate and SYNB8802 transit through the stomach, small intestine, and colon are modeled according to a physiological function of chyme progression. Oxalate absorption and consumption occur sequentially in as chyme transits through the three compartments.
2. SYNB8802 begins in the stomach and empties into the small intestine during the first 4 h post‐meal. From 4 to 8 h post‐meal, SYNB8802 empties from the small intestine into the colon. Approximately 36 h post‐meal, SYNB8802 begins to empty from the colon via fecal excretion.
3. ISS connects in vitro strain activity knowledge to host and disease biology. The strain‐side model (green) simulates the consumption of oxalate by SYNB8802 within the gastrointestinal physiology. The host‐side model (overall schematic) simulates the impact of consumption by SYNB8802 on the distribution of oxalate throughout the body.