Figure 4. In silico simulation (ISS) strain activity and pH inhibition model.

1. Michaelis–Menten model of strain activity kinetics. Vmax defines the maximal strain activity velocity (consumption rate of oxalate by SYNB8802). Km defines the oxalate concentration at which half‐maximal strain activity velocity occurs. Vmax and Km were determined through in vitro simulation.
2. Simulated gastric pH as a function of time following a solid meal (dark blue). This function is a power exponential decay with a half‐life of 110 min and a shape parameter equal to 1.81. Likelihood of time spent in the stomach based on gastric residence time distribution (light blue). The distribution is truncated to a maximum of 4 h and the median gastric residence time is 110 min.
3. Simulated normalized SYNB8802 activity in the stomach as a function of time.
4. Simulated normalized SYNB8802 activity in the small intestine as a function of time previously spent in the stomach. Function is equivalent to gastric function with an upper limit imposed based on intestinal pH.