-
A
Representative images of mice treated with DEBIO‐0932 (160 mg/kg, o.g.) starting at 7 days after intracardiac inoculation of H2030‐BrM cells. Treatment was given daily during the first week and every 48 h during the two following weeks.
-
B, C
Quantification of metastatic progression as measured by in vivo BLI of head (B) and extracranial region (C) of animals. Values are shown as mean ± s.e.m. (n = 9 vehicle and n = 9 DEBIO‐0932‐treated mice, 2 independent experiments). P value was calculated using two‐tailed t‐test (P values: *P < 0.05, ***P < 0.001).
-
D
Representative images of brains and thorax from vehicle and DEBIO‐0932‐treated mice at the endpoint of the experiment.
-
E, F
Quantification of ex vivo BLI of brains (E) and thoracic regions (F) at the endpoint of the experiment. Values are shown in box‐and‐whisker plots where every dot represents a different animal and the line in the box corresponds to the median. The boxes go from the upper to the lower quartiles, and the whiskers go from the minimum to the maximum value (n = 9 vehicle and n = 9 DEBIO‐0932‐treated mice, 2 independent experiments). P value was calculated using two‐tailed t‐test.
-
G
Kaplan‐Meier curve comparing overall survival of vehicle and DEBIO‐0932‐treated mice starting 7 days post‐intracardiac injection (n = 10 mice treated with vehicle and n = 11 mice treated with DEBIO‐0932). P value was calculated using log‐rank (Mantel‐Cox) test. The arrow indicates when the treatment was initiated.
-
H
Schema showing the individualized therapy of mice receiving surgery + DEBIO‐0932 during the treatment period starting 3 days after surgery. Each row represents a mouse receiving DEBIO‐0932 (green) o not (white) (n = 11 surgery + DEBIO‐0932‐treated mice, 2 independent experiments). Gray squares indicate decease of the corresponding animal.
-
I
Graph showing survival of mice treated with surgery + vehicle or surgery + DEBIO‐0932. The graph represents each mouse with a bar only if the survival is above the median of the group receiving surgery + vehicle (60.5 days) (n = 8 surgery + vehicle and n = 11 surgery + DEBIO‐0932‐treated mice, 2 independent experiments). P value was calculated using two‐tailed t‐test.
