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Gene Set Enrichment Assay (GSEA) with significantly enriched gene sets to the high-Treg CRC patients in the TCGA and GSE39582 cohorts. Cancer aggravating gene sets (EMT, KRAS signaling up, hypoxia, TGF-β signaling, TNF-α signaling via NFkB, and coagulation) with false discovery rate (FDR) and normalized enrichment score (NES). As recommended by the GSEA software, we defined statistical significance by an FDR of less than 0.25.
