Gene Set Enrichment Assay (GSEA) of immune response-related gene sets by high vs. low Treg CRC in the TCGA and GSE39582 cohorts. Interferon (IFN)-γ response, IL2/STAT5 signaling, IFN-α response, allograft rejection, complement, IL6/JAK/STAT3 signaling, and inflammatory response gene sets with false discovery rate (FDR) and normalized enrichment score (NES). As recommended by the GSEA software, FDR of 0.25 defined statistical significance.