Figure 11.

The activation of CD8⁺ lymphocytes as well as secretion of IFN-γ and IL-2 can be suppressed by CDDP-treated ESCC cells in the co-culture system while the addition of anti-PD-1 antibody prevent this process. (A and B) ESCC cells/PBMCs co-culture system was established. After co-culturing, the levels of IL-2 and IFN-γ in supernatants were measured by ELISA. Data from three independent experiments were shown (means ± SEM). (C-F) PBMCs from K30 cells/PBMCs co-culture system (C) and TE-1 cells/PBMCs co-culture system (D) were collected and stained with CD8-PB and CD25-PE, the ratio of CD25⁺ cells in CD8⁺ lymphocytes were detected by flow cytometry. Percentages of CD25⁺ cells in CD8⁺ lymphocytes in K30 cells/PBMCs co-culture system (E) and TE-1 cells/PBMCs co-culture system (F) from three independent experiments were shown (means ± SEM). **P<0.01, ***P<0.001 and ****P<0.0001.