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. 2022 Feb 15;12(2):537–548.

Figure 6.

Figure 6

Effects of Ahrr knockdown and EPAS1/VEGFD signal axis on LNM in HNC tumors in vivo. A. Transplanted tumor of Ahrr -/-, Ahrr +/+ and Ahrr +/- HNCs were harvested as described in Materials and Methods. Frozen sections of shNT and shAhrr#3 tumors were stained for lymphatic vessels with anti-LYVE-1 antibody (green), followed by staining with DAPI (blue). Scale bar 100 μm. B, C. Quantification of lymphatic vessels in shNT and shAhrr#3 transplanted tumors. Data were expressed as mean area density of LYVE-1-positive pixels per microscopic field (mean ± SD). **P<0.05 versus shNT. Frequency of LNM in HNCs (n=20), and shNT (n=19) and shAhrr#3 (n=17) tumor-bearing mice.