KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| anti-HSV-1 ICP4 Immediate Early Protein [10F1], mouse monoclonal | Abcam | cat #ab6514; RRID:AB_305537 |
| anti-HSV-1 ICP0 [5H7], mouse monoclonal | Abcam | cat #ab6513; RRID:AB_305536 |
| anti-HSV ICP5, mouse monoclonal | Virusys Corporation | cat #HA018; RRID:AB_2713935 |
| anti-VSV-G tag [P5D4], mouse monoclonal | Abcam | cat #ab50549; RRID:AB_883494 |
| anti-VacV I3 | Dr. David Evans (University of Alberta, Edmonton, Canada) | N/A |
| anti-VacV G8 | Dr. Paula Traktman (Medical University of South Carolina, Columbia, SC, USA) | N/A |
| anti-VacV A25 | Dr. Yan Xiang (University of Texas Health Sciences Center, Austin, TX, USA) | N/A |
| anti-VacV A27 | Life Technologies | cat #J97Q |
| anti-IFNβ antibody | Millipore-Sigma | cat #ab1431; RRID:AB_90638 |
| anti-Flag DYKDDDDK Tag [D6W5B] for IF, rabbit monoclonal | Cell Signaling Technology | cat #15009; RRID:AB_2798687 |
| anti-DNA-PK, mouse monoclonal | Cell Signaling Technology | cat #12311, 3H6; RRID:AB_2797881 |
| anti-IRF9 | Cell Signaling Technology | cat #76684, D2T8M; RRID:AB_2799885 |
| anti-HSPA8, rat | Enzo | cat #ADI-SPA-815-J, 1B5 |
| anti-tyrosinated tubulin, rat | Dr. Gregg Gunderson (Columbia University, New York, NY, USA) | N/A |
| anti-GFP | Abcam | cat #ab13970; RRID:AB_300798 |
| anti-pIRF3, phospho S386 | Abcam | cat #ab76493: RRID:AB_1523836 |
| anti-FEZ1, rabbit monoclonal | Cell Signaling Technology | cat #42480, D9R8Q; RRID:AB_2799222 |
| anti-FEZ1, mouse monoclonal | Abnova | cat #H00009638-B02P |
| anti-PKR | Cell Signaling Technology | cat #12297, D7F7; RRID:AB_2665515 |
| anti-MxA | Cell Signaling Technology | cat #37849, D3W7I; RRID:AB_2799122 |
| anti-MxB | Cell Signaling Technology | cat #43924, E7Y8H |
| anti-ISG56 | Cell Signaling Technology | cat #14769, D2X9Z; RRID:AB_2783869 |
| anti-IRF3 | Cell Signaling Technology | cat #11904, D6I4C; RRID:AB_2722521 |
| anti-IRF7 | Cell Signaling Technology | cat# 72073, D8V1J |
| anti-Lamin B1 | Cell Signaling Technology | cat #13435, D9V6H; RRID:AB_2737428 |
| anti-NF-kB | Cell Signaling Technology | cat #8242, D14E12; RRID:AB_10859369 |
| anti-GAPDH | Santa Cruz.Antibodies | cat #sc-25778; RRID:AB_10167668 |
| anti-phospho-S58-FEZ1 | Dr. John Jia En Chua (Max Planck Institute for Biophysical Chemistry, Gottingen, Germany) | N/A |
| anti Rabbit IgG, HRP | GE Healthcare UK | cat #NA934V |
| anti Mouse IgG, HRP | GE Healthcare UK | cat #NA931V |
| anti Rat IgG, HRP | Invitrogen | cat #PA1-28664 |
| anti-Rat secondary, Alexa 647 | Jackson ImmunoResearch | cat #712-605-150; RRID:AB_2340693 |
| anti-Mouse secondary, Alexa 488 | Life Technologies | cat #A21202; RRID:AB_141607 |
| anti-Rabbit secondary, Alexa 488 | Life Technologies | cat #A21206; RRID:AB_2535792 |
| Bacterial and virus strains | ||
| Escherichia coli (E. coli) DH5α strain | Thermo Fisher Scientific | cat #18265017 |
| HSV-1 | Dr. Ian Mohr (NYU-Lagone, New York, NY, USA) | N/A |
| VACV (WR) | Dr. Stewart Shuman (Sloan-Kettering Institute, New York NY, USA) | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| ProteaseMAX | Promega | cat #V207A |
| Trypsin Gold | Promega | cat #V528A |
| Pierce Spin Columns with C18 resin | Thermo Fisher Scientific | cat #89879 |
| microBCA protein assay kit | Thermo Fisher Scientific | cat #23235 |
| nanoViper trap column | Thermo Fisher Scientific | cat #164535 |
| nanoViper analytical column | Thermo Fisher Scientific | cat #164942 |
| G-sepharose | GE Healthcare | cat #17-0618-01 |
| Lipofectamine 2000 Transfection Reagent | Thermo Fisher Scientific | cat # 11668019 |
| CT DNA | Sigma | cat #D4764 |
| Nu-7441 DNA-PK inhibitor | SelleckChem | cat #S2638 |
| Polybrene | Santa Cruz Biotechnology | cat #sc-134220 |
| FluroSave Reagent | MilliporeSigma | cat #345789 |
| Hoechst 33342 | Thermo Fisher Scientific | cat #62249 |
| PlasmoTest mycoplasma detection kit | InvivoGen | Cat #rep-pt1 |
| Lipofectamine RNAiMAX Transfection Reagent | Thermo Fisher Scientific | cat #13778030 |
| QUANTI-Blue | InvivoGen | cat # rep-qbs |
| QUANTI-Luc | InvivoGen | cat #rep-qlc1 |
| Cas9 protein | UC-Berkeley Macrolab | N/A |
| SF Nucleofector solution | Lonza | cat #V4XC-2012 |
| GFP-Trap agarose | ChromoTek | cat #gta-100 |
| Opti-Mem medium | Fisher Scientific | cat #31985070 |
| DMEM | Fisher Scientific | cat #MT15013CV |
| RPMI 1640 | Fisher Scientific | cat #11875093 |
| 0.1% Gelatin solution | Millipore | cat #ES-006-B |
| Amicon Ultra-15 Ultracel-3K centrifugal filters | Millipore | cat #UFC500324 |
| Human IFN beta ELISA kit | PBL Assay Sciences | cat #41100-1 |
| Human IFN-b | EMD Millipore, Millipore Sigma | cat #IF014 |
| NE-PER nuclear and cytoplasmic extraction reagent | Thermo Fisher Scientific | cat #78833 |
| EcoRI-HF | New England BioLabs | cat #R3101S |
| SbfI-HF | New England BioLabs | cat # R3642S |
| NotI-HF | New England BioLabs | cat #R3189S |
| Pierce Protease Inhibitor Mini Tablets, EDTA-free | Thermo Fisher Scientific | cat #A32955 |
| Experimental models: Cell lines | ||
| CHME3 | Dr. Marc Tardieu (Universite Paris Sud, France) | N/A |
| Human: Normal Human Dermal Fibroblasts (NHDFs) | Lonza | cat #CC-2509 |
| African green monkey: BSC-40 | Dr. Ian Mohr (NYU-Lagone, New York, NY, USA) | N/A |
| African green monkey: Vero | Dr. Ian Mohr (NYU-Lagone, New York, NY, USA) | N/A |
| HEK-293T (293T) | ATCC | cat #CLR-3216 |
| THP1-Dual cell line | InvivoGen | cat #thpd-nfis |
| THP1-Dual KO-STING cell line | InvivoGen | cat #thpd-kostg |
| Oligonucleotides | ||
| See Table S2 for oligonucleotide information | N/A | |
| Recombinant DNA | ||
| pQCXIN | Clontech | cat #631514 |
| pQCXIN-Flag | (Malikov et al., 2015) | N/A |
| pQCXIN-FEZ1-Flag | (Malikov et al., 2015) | N/A |
| pQCXIN-FEZ1-S58A-Flag | (Malikov et al., 2015) | N/A |
| pQCXIN-eGFP | This paper | N/A |
| pQCXIN-eGFP-FEZ1 | This paper | N/A |
| pQCXIN-eGFP-FEZ1-S58A | This paper | N/A |
| pPM-C-HA-HSPA8 | Applied Biological Materials | Cat # BC016179 |
| pQCXIN-HSPA8-Flag | This paper | N/A |
| pQCXIN-eGFP-HSPA8 | This paper | N/A |
| pCMV-intron | Dr. Stephen P. Goff (Columbia University, New York, NY, USA) | N/A |
| pVSV-G | Dr. Stephen P. Goff (Columbia University, New York, NY, USA) | N/A |
| Software and algorithms | ||
| FIJI (image J installation) | Open source | http://fiji.sc RRID:SCR_002285 |
| MetaMorph Microscopy Automation and Image Analysis Software | Molecular Devices | RRID:SCR_002368 |
| GraphPad Prism (Version 7) | Graphpad | RRID:SCR_002798 |