Figure 3.

Cellular localization of the six mutants of SLC26A4 in 293T cells detected by confocal microscopy. Mutant p.S49R, p.T485M, p.D661E of SLC26A4 show strong membrane fluorescence in 293T cells, which is similar to the WT of SLC26A4 expressed in the 293T cells, and there was no obvious cytoplasmic retention. Mutants p.I363L, p.R409H, and p.H723R reduce the localization on the cell membrane, and p.R409H, p.H723R show obvious cytoplasmic retention, p.I363L is less. All mutant plasmids were homozygous. Scale: 10 μm

WT: wild-type