Figure 4.

The effect of the mutations in SLC26A4 on iodine transport capacity in 293T cells. Intracellular iodide accumulation in 293T cells after co-transfection with sodium-iodide symporter (NIS) and wild-type (WT) or the mutant SLC26A4-pEGFP-N2 plasmids were detected by γ-counter. Intracellular iodide accumulation in 293T cells after co-transfection with NIS and mutants palsmids from the p.I363L, p.R409H, p.T485M, p.D661E and p.H723R variants were significantly increased, compared to those co-transfected with NIS and WT (pendrin) SLC26A4-pEGFP-N2 plasmid, indicating that these mutants reduced iodide efflux mediated by SLC26A4. All mutant plasmids were homozygous. The data are shown as mean±standard error for three independent experiments. Statistical analysis used Welch’s t-test

ns: No statistical difference, ***p<0.001