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. 2022 Feb 21;13:794210. doi: 10.3389/fphys.2022.794210

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Copyright © 2022 Shi, Zhao, Xue, Li, Yao, Wang, Wang, Lu and Shi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The validation procedure of the study design. Human blastocysts were donated by in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) patients with preserved surplus embryos after giving birth to live healthy babies. We then validated non-invasive chromosome screening (NICS) using spent culture medium (SCM) versus blastocoel fluid (BF) as a source of DNA. In each embryo, we obtained the chromosome ploidy results from the inner cell mass (ICM), trophectoderm biopsy (TE), SCM, and BF after whole-genome amplification (WGA) and library preparation by NICSInst. Copy number variation (CNV) was determined by ChromGo Analysis Software.