TABLE 1.

The diagnostic performance of NGS of spent culture medium and blastocoel fluid DNA for aneuploidy/mosaicism.

	Total			Consistency with inner cell mass			Consistency with whole blastocyst
Assay	SCM	BF	P	SCM	BF	P	SCM	BF	P
Specificity	80.2% (81/101)	61.4% (62/101)	0.005	85.0% (51/60)	60.0% (36/60)	0.004	73.2% (30/41)	63.4% (26/41)	0.476
Sensitivity	91.5% (43/47)	87.2% (41/47)	0.738	93.1% (27/29)	86.2% (25/29)	0.670	88.9% (16/18)	88.9% (16/18)	>0.999
NPV	95.3% (81/85)	91.2% (62/68)	0.487	96.2% (51/53)	90.0% (36/40)	0.433	93.8% (30/32)	92.9% (26/28)	>0.999
PPV	68.3% (43/63)	51.3% (41/80)	0.042	75.0% (27/36)	51.0% (25/49)	0.044	59.3% (16/27)	51.6% (16/31)	0.749

NGS, next generation sequencing; BF, blastocoel fluid; SCM, spent culture medium; NPV, negative predictive value; PPV, positive predictive value.