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. 2022 Mar 7;22:102. doi: 10.1186/s12870-022-03487-y

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© The Author(s) 2022, corrected publication 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — SOS3 and ABI5 expression is activated in the presence of SmAP2-17 as measured by the luciferase reporter assays. a Schematic diagrams of the putative AP2/ERF binding elements in the 2000-bp promoter region of the five candidate downstream genes. b Left panel: Schematic diagrams of effector and reporter plasmids used in transient transactivation assays: top, schematic diagrams of control (EV, empty vector) and effector (RV, recombination vector); bottom, schematic diagrams of reporter constructs. The promoter sequences from five candidate downstream genes were cloned upstream of the LUC reporter gene. The REN located downstream of the 35S promoter was used as an internal control. Right panel: The results showed that five candidate downstream genes, SOS3 and ABI5, were regulated by SmAP2-17. Relative activity was calculated as LUC/REN, but was normalized to the reporter only. Data represent the mean ± SD of three biological replicates. *P < 0.05 and **P < 0.01 by Student’s t-test