Table 2.
antibody used as target and reference used in immunohistochemistry and Western Blot reactions
| Antibody | Company | Accession no. | Concentration of antibodies in immunohistochemistry | Concentration of antibodies in Western Blot |
|---|---|---|---|---|
| Vimentin (D21H3) XP® Rabbit mAb | Cell Signaling Technology | #5741 | 1:200 | |
| LDHA (C4B5) Rabbit mAb | Cell Signaling Technology | #3582 | 1:200 | 1:1000 |
| PKM2 (D78A4) XP® Rabbit mAb | Cell Signaling Technology | #4053 | 1:500 | 1:1000 |
| HK2 Rabbit pAb (A0994) | ABclonal Technology | #3099 | 1:200 | 1:1000 |
| Bax (D3R2M) Rabbit mAb | Cell Signaling Technology | #14796 | 1:100 | |
| Bcl-2 Rabbit mAb | ABclonal Technology | #19693 | 1:100 | |
| SignalStain® Boost IHC Detection Reagent (HRP, Mouse) | Cell Signaling Technology | #8125 | 1:200 | |
| Biotin-conjugated Affinipure Goat Anti-Rabbit IgG(H + L) | Protein Tech Group Inc., USA | SA00004-2 | 1:200 | |
| HRP-conjugated Streptavidin | Protein Tech Group Inc., USA | SA00001-0 | 1:200 | |
| alpha Tubulin Monoclonal Antibody (236-10,501) | Thermofisher scientific | A11126 | 1:3000 | |
| HRP-conjugated affinipure goat anti-mouse IgG (H + L) | Protein Tech Group Inc., USA | SA00001-1 | 1:5000 | |
| goat anti-rabbit IgG (H + L) | Protein Tech Group Inc., USA | SA00001-2 | 1:5000 |