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aReagents and conditions: the reactions were conducted with 20 equiv oxazolines in PBS buffer; (a) in the case of the biotin tag, one additional portion (10 equiv) of oxazoline 39 was added after 60 min; (b) in the case of the fluorescent tag, four more portions (10 equiv each) of oxazoline 40 were added every 60 min to push the reaction to completion.
