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. Author manuscript; available in PMC: 2022 Nov 19.
Published in final edited form as: ACS Chem Biol. 2021 Sep 27;16(11):2502–2514. doi: 10.1021/acschembio.1c00597

Scheme 6. Synthesis of Structurally Defined ADCs with Different DARs by Click Chemistrya.

Scheme 6.

aReagents and conditions: the azido-tagged antibodies (final concentration 2 mg/mL) were incubated with DBCO-PEG5-VC-PAB-MMAE (3.3–5.0 equiv/per azido group) in DMSO/50 mM PB (3:7, v/v) at room temperature for 8–24 h.