Table 1.

Screening of the Transglycosylation Conditions

ENGases	transglycosylation				product hydrolysis
	oxazoline (equiv)a	enzyme (w/w) (%)	time (h)	conversion yield (%)
Endo-S2	20	0.1	1	98	<1%c
Endo-S2 D184M	20	1	2	70	no
Endo-S	20 + 20	1	2	65	no
Endo-S D233Q	20	10	3	15	no
Endo-F3	20	10	3	<5	d
Endo-F3 D165A	20	10	3	<5	d
Endo-A	20	10	3	0 (<5%b)	d
Endo-D	20	10	3	0 (10%b)	no
Endo-CC	20	10	3	0 (<5%b)	d

^aBased on each glycosylation site.

^bNonfucosylated antibody (GlcNAc-Herceptin) was used as the acceptor, as these three enzymes do not recognize the Fuca1,6GlcNAc-antibody as the acceptor.

^cAfter 6 h at room temperature.

^dNot determined.