AKT degrader 20 suppresses the tumor growth in vivo in a PC3 cell line xenograft mouse model. (A) Nude mice (Foxn1nu, athymic nude) bearing established PC-3 xenografts were treated with vehicle (n = 6) or 20 (n = 7, 75 mg/kg, i.p., once daily) for 22 days. Data points represent mean tumor volume ±SE. (B) Body weights of mice at the beginning and the end of the in vivo studies. (C) Western blotting analysis of P-AKT and T-AKT protein levels, and downstream signaling inhibition (P-PRAS40) in the tumor samples isolated from the nude mice treated with vehicle or 20.