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. Author manuscript; available in PMC: 2023 Feb 24.
Published in final edited form as: J Med Chem. 2022 Feb 4;65(4):3644–3666. doi: 10.1021/acs.jmedchem.1c02165

Figure 8.

Figure 8.

Compounds 20 and 35 induced AKT degradation and inhibited the downstream signaling in a time-dependent and reversible manner. (A,B) PC3 cells treated with DMSO (24 h), AZD5363 (24 h), 20, or 35 at 1 μM were collected at the indicated time points for western blotting analysis. (C,D) Western blots of T-AKT, P-AKT, P-PRAS40, and P-S6 and β-actin post-treatment of PC3 cells with DMSO, AZD5363, 20, or 35 at 1 μM for 24 h at the indicated time. The compounds were washed out with fresh medium after the 24 h treatment.