Figure 4.
RT-qPCR for the circular form of the bacteriophage and the phage-free chromosomal integration site (attB). The circular form (cf) of the bacteriophage and phage-free chromosomal site (attB) were detected by PCR. Isolates are regarded as IEC+ and IEC- according to WGS data. Results of three independent experiments performed as duplicates. The comparison of two data groups were analyzed by Mann–Whitney U test (one-tailed, confidence intervals 95%) with **P < 0.01.