Figure 1.

hBEC characterization in steatotic and healthy livers

(A) Left: oil red O staining in healthy and steatotic human rejected livers. Scale bars, 100 μm. Right: quantification of the percentage of oil red O. ^∗ denotes p < 0.05 (mean ± SEM), Student’s t test between healthy (n = 3) and steatotic livers (n = 3).

(B) Isotype control, healthy, and steatotic human livers stained for SOX9, EpCAM, and K19. Scale bars, 250 μm. Far right: total pixel quantification expressed as percentage in healthy (H) and steatotic (S) human livers. Steatotic livers have significantly increased levels of SOX9, EpCAM, and K19 in comparison with healthy livers. ^∗ denotes p < 0.05 (mean ± SEM), Student’s t test (n = 3).

(C) Representative gating strategy for isolation of EpCAM+/CD24+/CD133+ hBECs from whole healthy and steatotic liver digests. hBECs were defined as live, non-hematopoietic single cells that expressed EpCAM and CD24. This population was further subdivided into CD133− and CD133+ fractions.

(D) Fluorescence minus one (FMO) control staining for setting positive staining gates for the isolation of EpCAM+/CD24+/CD133+ hBECs.