Evaluation of gene expression (U.A.) of insulin signaling pathway mediators (n = 3–4) and peroxisome proliferator-activated receptor gamma (PPARγ, n = 4) in the liver of rats fed a high-fat diet (30% fat, FAT) or a control diet (AIN-93, CT) for 13 weeks and submitted to physical training (PT) or maintained sedentary (SED) during the last 6 weeks of the diet. (A) Insulin receptor (IR), (B) Insulin receptor substrate 1 (IRS-1), (C) Insulin receptor substrate 2 (IRS-2) (D) serine/threonine kinase 2 (AKT-2), (E) glucose transporter type 4 (GLUT4), (F) peroxisome proliferator-activated receptor gamma (PPARγ). *P < 0.05 compared to CT-SED group. #P < 0.05 compared to FAT-SED group (two-way ANOVA followed by Bonferroni post-test).