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. 2017 May 22;22(6):501–507. doi: 10.1080/13510002.2017.1329917

Figure 2.

Figure 2.

Pter activated Nrf2/ARE via PI3K. After transfection with luciferase reporter plasmid for 24 h, HaCaT cells were pretreated for 1 h with LY294002 prior to Pter treatment. (A) Luciferase activity data are presented as percentage of the untreated control ± SD (n = 6); ns = P > 0.05, ***P < 0.001. (B) The indicated proteins were detected by western blotting, with laminA as the loading control. (C) The levels of phosphorylated Nrf2 were quantified by densitometric scanning. Values are mean ± SD (n = 3); **P < 0.01, ***P < 0.001. (D) Cell viability in HaCaT cells treated as indicated. Data are presented as percentage of the untreated control ± SD (n = 6); ns = P > 0.05, *P < 0.05, ***P < 0.001.