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. Author manuscript; available in PMC: 2023 Jan 20.
Published in final edited form as: Nanoscale. 2022 Jan 20;14(3):797–814. doi: 10.1039/d1nr06143g

Figure 2.

Figure 2.

IGF-1 concentration optimization effect on the differentiation of C2C12 cells encapsulated in 4% GelMA hydrogel. a) Myosin heavy chain (purple) staining at day 14 and day 21, demonstrating the differentiation of myoblasts within GelMA hydrogels exposed to different IGF-1 levels. b) Quantification of the number of myotubes from varying concentrations of IGF-1. Higher levels of IGF-1 led to higher numbers of multinucleated myotubes. c) The quantification of myotube thickness. The highest concentration of IGF-1 (100 ng/mL) resulted in thicker, more mature myotubes compared to controls without IGF-1. Scale bars are 250 μm for all micrographs.