Figure 4.

CircRNA_Maml2 acts as a sponge for miR-93-3p in CT26.wt cells. (a) Expression of miRNAs after the overexpression of circRNA_Maml2 was detected by qRT-PCR. (b) Expression of miRNAs in the intestinal tissues of mice after severe burns was detected by qRT-PCR. (c) miR-93-3p was pulled down by using a circRNA_Mmal2 with a specific biotinylated MS2 tag. (d) The binding sites of circRNA_Maml2 and miR-93-3p were predicted by bioinformatics software. Schematic illustration of circRNA_Mmal2-WT and circRNA_Mmal2-Mut luciferase reporter vectors. Relative luciferase activities were investigated in CT26.wt cells after transfection with circRNA_Mmal2-WT or circRNA_Mmal2-Mut and miR-93-3p mimic or miR-NC. *p < 0.05, **p< 0.01, ***p < 0.001. Ad adenovirus, WT wild type, Mut mutant, qRT-PCR quantitative real-time polymerase chain reaction