Skip to main content
. 2022 Feb 28;78(Pt 3):135–142. doi: 10.1107/S2053230X22002138

Table 1. Macromolecule-production information.

Source organism Chlamydia trachomatis (strain D/UW-3/Cx)
DNA source Dr Kevin Hybiske (University of Washington, USA)
Forward primer 5′-CTCACCACCACCACCACCATATGTCTAAAACACCATTATCCATAGC-3′
Reverse primer 5′-ATCCTATCTTACTCACTTACATAAAAAGATTGCAATAGTCTTCGT-3′
Expression vector pBG1861
Expression host E. coli BL21(DE3)R3 Rosetta cells
Complete amino-acid sequence of the construct produced MAHHHHHHMSKTPLSIAHPWHGPVLTRDDYESLCCYIEITPADSVKFELDKETGILKVDRPQKFSNFCPCLYGLLPKTYCGDLSGEYSGQQSNRENIKGDGDPLDICVLTEKNITQGNILLQARPIGGIRILDSEEADDKIIAVLEDDLVYGNIEDISECPGTVLDMIQHYFLTYKATPESLIQAKPAKIEIVGLYGKKEAQKVIRLAHEDYCNLFM