Table 1. Macromolecule-production information.
Source organism | Chlamydia trachomatis (strain D/UW-3/Cx) |
DNA source | Dr Kevin Hybiske (University of Washington, USA) |
Forward primer | 5′-CTCACCACCACCACCACCATATGTCTAAAACACCATTATCCATAGC-3′ |
Reverse primer | 5′-ATCCTATCTTACTCACTTACATAAAAAGATTGCAATAGTCTTCGT-3′ |
Expression vector | pBG1861 |
Expression host | E. coli BL21(DE3)R3 Rosetta cells |
Complete amino-acid sequence of the construct produced | MAHHHHHHMSKTPLSIAHPWHGPVLTRDDYESLCCYIEITPADSVKFELDKETGILKVDRPQKFSNFCPCLYGLLPKTYCGDLSGEYSGQQSNRENIKGDGDPLDICVLTEKNITQGNILLQARPIGGIRILDSEEADDKIIAVLEDDLVYGNIEDISECPGTVLDMIQHYFLTYKATPESLIQAKPAKIEIVGLYGKKEAQKVIRLAHEDYCNLFM |