| Method |
Vapor diffusion, sitting drop |
| Plate type |
96-well Compact 300, Rigaku |
| Temperature (K) |
287 |
| Protein concentration (mg ml−1) |
31 |
| Buffer composition of protein solution |
3 mM inorganic pyrophosphate, 25 mM HEPES pH 7.0, 500 mM NaCl, 5% glycerol, 2 mM DTT, 0.025% azide |
| Composition of reservoir solution |
2 M NaCl, 0.1 M Tris pH 8.5, 25%(v/v) PEG 3350 |
| Volume and ratio of drop |
0.4 µl protein plus 0.4 µl reservoir |
| Volume of reservoir (µl) |
80 |
| Composition of cryoprotectant solution |
2 M NaCl, 0.1 M Tris pH 8.5, 25%(v/v) PEG 3350, 15%(v/v) ethylene glycol |
