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| Method | Sitting drop |
| Plate type | Intelli-Plate 96-3 |
| Temperature (K) | 293 |
| Protein concentration (mg ml−1) | 17 |
| Buffer composition of protein solution | 20 mM Tris–HCl pH 7.5, 150 mM NaCl, 5 mM β-mercaptoethanol |
| Composition of reservoir solution | 0.4–0.6 M (NH4)H2PO4 |
| Volume and ratio of drop | 400 nl, 1:1 |
| Volume of reservoir (µl) | 50 |
