Table 2. Crystallization.
Values in parentheses are for the outer shell.
| PDB code | 7p6g | 7p6h | 7p6i | 7p6j |
|---|---|---|---|---|
| Method | Vapor diffusion, hanging drop | Vapor diffusion, hanging drop | Vapor diffusion, hanging drop | Vapor diffusion, hanging drop |
| Plate type | EasyXtal (15-well) | EasyXtal (15-well) | EasyXtal (15-well) | EasyXtal (15-well) |
| Temperature (K) | 293 | 293 | 293 | 293 |
| Protein concentration (µM) | 385 | 385 | 400 | 400 |
| Buffer composition of protein solution | 20 mM sodium phosphate buffer pH 6.5 | 20 mM sodium phosphate buffer pH 6.5, 1 mM cellotriose | 20 mM sodium phosphate buffer pH 6.5 | 20 mM sodium phosphate buffer pH 6.5, 1 mM cellotriose |
| Composition of reservoir solution | 0.1 M Tris, 17.5% PEG 600 pH 8 | 0.1 M Tris, 17.5% PEG 600 pH 7.4 | 0.1 M Tris, 17.5% PEG 600 pH 7 | 0.1 M Tris, 20.5% PEG 600 pH 7 |
| Volume and ratio of drop | 1:1 | 1:1 | 1:1 | 1:1 |
| Volume of reservoir (µl) | 500 | 500 | 500 | 500 |