Table 2. Ligand-binding properties.

Values are given with standard errors.

Ligand	AcAChBP, K d (nM)	α4β2 nAChR, K i † (nM)
1	9.8 ± 0.03	0.12 ± 0.020
2	60.0 ± 0.14	0.28 ± 0.010
3	29.0 ± 0.05	0.94 ± 0.070
4	33.0 ± 0.08	0.07 ± 0.002
5	30.0 ± 0.06	0.28 ± 0.030
6	9.8 ± 0.01	0.67 ± 0.280
Epibatidine	14.0‡	0.02 ± 0.001
Varenicline	342.0§	0.12 ± 0.002
Nicotine	835.0§	0.95¶

^†[³H]-Epibatidine competition assay, K _d = 0.02 nM (Ondachi et al., 2016 ▸).

^‡Tryptophan fluorescence-quenching assay (Hansen et al., 2005 ▸).

^§Isothermal titration calorimetry (Rucktooa et al., 2012 ▸).

^¶[³H]-Nicotine competition assay (Coe et al., 2005 ▸).