Table 2. Crystallization.

Method	Hanging-drop vapour diffusion
Plate type	Sample cups and siliconized cover glasses
Temperature (K)	293
Protein concentration (mg ml−1)	10
Buffer composition of protein solution	20 mM Tris–HCl pH 7.5, 100 mM NaCl, 5 mM DTT
Composition of reservoir solution	0.15–0.20 M potassium acetate, 20%(w/v) PEG 3350
Volume and ratio of drop	1 µl:1 µl
Volume of reservoir (µl)	400