Figure 1.

Generalized beam alignment and image acquisition setup for application in synchrotron facilities and tabletop sources. CAD design (a). Actual setup as assembled in the Berkeley ALS 8.3.2 beamline hutch (b). Our custom detector operates using a scintillator (A¹) to convert X-rays (A²) passing through a sample (A³) into visible light. The fluorescence image is then focused by the objective lens (A⁴) onto the CMOS sensor surface (A⁵). Commercially available Newport stages (A⁶–A⁸) were utilized to adjust the camera relative to the focal point, and to translate the detector through three-dimensional space (15 cm vertical travel and 10 cm horizontal travel in any direction) for optimal alignment with any synchrotron- or tabletop-generated beam. Projections (A⁹) acquired by the detector are saved using the Harvester Python library and later reconstructed with the TomoPy toolbox in Python.