Table 1.
Primer sequences.
| No. | Sequence (5′-3′) | Orientation | Description |
|---|---|---|---|
| 1 | CGATCTCTTGTAGATCTGTTCTCT | Forward | RT–PCR, qRT–PCR for sgRNA3 (Fig. 1C) |
| 2 | GTCTTCCTTGCCATGTTGAGT | Reverse | |
| 3 | GAAGGTGAAGGTCGGAGTC | Forward | RT–PCR, qRT–PCR for GAPDH mRNA |
| 4 | GAAGATGGTGATGGGATTTC | Reverse | |
| 5 | GAGCTGGTGGCCATAGTTACGGCGCCGATC | Forward | Subcloning, PCR amplification of F1 |
| 6 | GTTTCAAGAGTGCGGGAGAAAATTGATC | Reverse | |
| 7 | ACGATCAATTTTCTCCCGCACTCTTGAAAC | Forward | Subcloning, PCR amplification of F2 |
| 8 | CACTTTTACACTTTTTAAGCACTGTCTTTG | Reverse | |
| 9 | CAAAGACAGTGCTTAAAAAGTGTAAAAGTG | Forward | Subcloning, PCR amplification of F3 |
| 10 | CAAACACGGTTTAAACACCGTGTAACTATG | Reverse | |
| 11 | CATAGTTACACGGTGTTTAAACCGTGTTTG | Forward | Subcloning, PCR amplification of F4 |
| 12 | GACAAAACCCACTTCTCTTGTTATGACTGC | Reverse | |
| 13 | GCAGTCATAACAAGAGAAGTGGGTTTTGTC | Forward | Subcloning, PCR amplification of F5 |
| 14 | GCATTTTCATACAAAAAAAAGAACAAAGAC | Reverse | |
| 15 | GTCTTTGTTCTTTTTTTTGTATGAAAATGC | Forward | Subcloning, PCR amplification of F6 |
| 16 | TAATGTGTCACAATTACCTTCATCAAAATG | Reverse | |
| 17 | AGGCATTTTGATGAAGGTAATTGTGACACATTAAAAG | Forward | Subcloning, PCR amplification of F7 |
| 18 | AACTATATCTGCTGTCGTCTCAGGCAATGC | Reverse | |
| 19 | CTTTTGTACTGTAAATGCATTGCCTGAGAC | Forward | Subcloning, PCR amplification of F8 |
| 20 | TAAACAGGAAACTGTGGCGCGCCAGTGTTA | Reverse | |
| 21 | TGTTAACAACTAACACTGGCGCGCCACAGT | Forward | Subcloning, PCR amplification of F9 |
| 22 | TAAAAGGTAAACAGGAAACTGTGGATCCTC | Reverse | |
| 23 | GATCGGCGCCGTAACTATGGCCACCAGCTC | Forward | PCR amplification of F10 |
| 24 | ACGAGTTCTTCTGAGGATCCACAGTTTCCT | Reverse | |
| 25 | AAATTCCCTCGAGGACAAGGCGTTC | Forward | PCR amplification of the N gene with KpnI and XhoI restriction sites for cloning into pBluescriptII |
| 26 | TTTTGGTACCACGTTCCCGAAGGTGTG | Reverse |