Evaluation of Cts L inhibition by the all-amide and thioamide peptidesa.
| Peptide | K I (μM) | α | αK I or K′I (μM) |
|---|---|---|---|
| K3A | 3.05 ± 0.64 | 1.91 ± 0.92 | 5.81 ± 3.06 |
| KS3A | 0.60 ± 0.15 | 2.18 ± 1.35 | 1.30 ± 0.87 |
| R3A | 1.68 ± 0.49 | 1.33 ± 0.78 | 2.23 ± 1.47 |
| RS3A | 0.52 ± 0.12 | 1.83 ± 0.99 | 0.95 ± 0.56 |
| RS1A | 1.11 ± 0.22 | 1.61 ± 0.69 | 1.79 ± 0.85 |
| RS1A* | 13.23 ± 6.89 | 1.71 ± 1.89 | 22.58 ± 27.61 |
a
Data was obtained by fitting to the mixed inhibition model that allows us to simultaneously determine the KI and the mechanism of inhibition using the output “alpha” (α) in GraphPad Prism 8 software.56,57 Detailed analysis are described in the ESI.