Skip to main content
. 2022 Feb 28;11:e71965. doi: 10.7554/eLife.71965

Figure 4. In dlx5/6 mice, a monosynaptic GABAergic input inhibits spiking in cells with a muscarinic response.

Figure 4.

(A) Two representative traces (scale bar 100 pA) showing spiking activity in control condition and while basal forebrain (BF) axons were photostimulated with a single flash per episode every 2 s (blue arrow). Middle: corresponding raster plot. Photostimulations started at episode 31 (blue line). Bottom: cumulative peri stimulus time histogram (PSTH) (bin size 20 ms) in the same cell for the trials with a photostimulation (blue arrow). (B) Average firing rate per bin (20 ms) and per episode for 17 cells (± SEM, gray bars) while BF axons were photostimulated every 2 s with a single flash (at blue arrow). (C) Average firing frequency per episode (2 s each) for 13 cells recorded in artificial cerebrospinal fluid (ACSF) in control condition (no light, episodes 1–30) and during photostimulation of the BF afferents once per episode (31–60). (D–F) Same as in (A–C) in the presence of 6-nitro-7-sulfamoylbenzo[f]quinoxaline-2,3-dione (NBQX) (10 µM), D-2-amino-5-phosphonopentanoic acid (D-AP5) (50 µM),, and mecamylamine (MECA, 50 µM). Traces, raster plot, and PSTH in (D) are from the same cell as in (A). (G–I) Same as in (A–C) when gabazine (GBZ, 5 µM) was added to the cocktail of blockers. Traces, raster plot, and PSTH in (G) are from the same cell as in (A) and (D).