Table 2. Percentage of ATAC-Seq peaks that overlap with the TSS±500 bp by at least 1 bp.

	Top 100 genes expressed in whole muscle but not in myofiber			Top 50 genes expressed in whole muscle but not in myofiber			All genes expressed in whole muscle tissue			All genes in the genome
	Number of overlapping peaks	Total number of peaks	% overlapping peaks	Number of overlapping peaks	Total number of peaks	% overlapping peaks	Number of overlapping peaks	Total number of peaks	% overlapping peaks	Number of overlapping peaks	Total number of peaks	% overlapping peaks
Uninjured_Fiber	12	19,704	0.0609013	3	19,704	0.0152253	7,865	19,704	39.915753	12,995	19,704	65.951076
Injured_Fiber	65	47,112	0.1379691	12	47,112	0.0254712	14,259	47,112	30.266174	26,198	47,112	55.607913
EDL_Whole_Muscle	198	60,719	0.3260923	65	60,719	0.1070505	18,419	60,719	30.334821	33,048	60,719	54.427774

Genes identified as being expressed solely in whole muscle but not in myofiber were retrieved from “High-resolution genome-wide expression analysis of single myofibers using SMART-Seq, JBC, Blackburn et al., 2019” and were defined as any gene with an expression of at least 10 RPM in the whole muscle RNA-seq, but 0 RPM in the single myofiber RNA-seq. All genes expressed in whole muscle tissue was defined as any gene that had an RPM value of at least 10 RPM from the whole muscle RNA-seq data by Blackburn et al., 2019 accessible through the GEO accession number GSE138591.