. 2022 Feb 28;2022:3298542. doi: 10.1155/2022/3298542

Table 2.

Flow cytometry analysis of lymphocyte populations from the spleen of AIR mice (n = 5–8). The results are expressed as a means percentage and standard deviation of 10,000 events acquired from 10⁷ cells/mL suspension.

Lymphocyte subsets	Days	Melanoma
		B16F10		S91		Control
		AIRmax	AIRmin	AIRmax	AIRmin	AIRmax	AIRmin
	7	n = 7	n = 5	n = 5	n = 5	n = 6	n = 5
CD3⁺		30.62 ± 5.64	22.83 ± 2.18	24.66 ± 6.23	27.57 ± 5.21	24.84 ± 4.13	28.29 ± 1.77
CD4⁺		20.60 ± 4.39	14.41 ± 0.90	15.76 ± 0.87	17.16 ± 2.91	14.96 ± 1.61	17.57 ± 1.66
CD8⁺		15.13 ± 1.66	12.11 ± 3.98	10.47 ± 1.85	11.40 ± 1.72	16.20 ± 3.20	9.53 ± 4.69
CD4⁺/CD25⁺		3.92 ± 0.58^A	2.89 ± 0.57^B	1.48 ± 0.21^B	2.94 ± 0.66^A	2.87 ± 0.90	1.99 ± 1.35
CD3⁺/CD49b⁺		2.60 ± 0.40	2.01 ± 0.23	1.21 ± 0.62	1.62 ± 0.23	2.02 ± 0.56	1.77 ± 0.26
CD49b⁺		6.29 ± 0.84	5.90 ± 1.54	3.85 ± 0.85	4.18 ± 1.45	6.01 ± 1.14	6.30 ± 1.90
	14	n = 8	n = 5	n = 8	n = 5	n = 8	n = 5
CD3⁺		32.47 ± 4.65	22.24 ± 2.54	36.86 ± 9.32	27.64 ± 4.56	39.55 ± 5.46^A	25.89 ± 4.28^B
CD4⁺		20.25 ± 2.58	15.04 ± 1.40	23.13 ± 4.96	18.23 ± 3.58	23.58 ± 2.92	16.76 ± 4.48
CD8⁺		12.46 ± 3.34	6.72 ± 1.17	14.38 ± 4.24^A	8.07 ± 0.90^B	15.20 ± 2.22^A	9.04 ± 2.24^B
CD4⁺/CD25⁺		2.64 ± 1.14^A	0.87 ± 0.38^B	2.64 ± 0.91	2.21 ± 1.29	2.90 ± 1.00	1.38 ± 0.86
CD3⁺/CD49b⁺		3.67 ± 0.99	3.23 ± 0.94	4.01 ± 0.87	3.22 ± 0.98	3.90 ± 1.22	3.94 ± 0.91
CD49b⁺		3.31 ± 0.51	4.61 ± 1.19	4.12 ± 0.85	4.58 ± 1.71	3.51 ± 1.01	3.98 ± 1.29
	30	n = 5	n = 5	n = 5	n = 5	n = 5	n = 5
CD3⁺		30.69 ± 4.71^a	24.12 ± 3.20	18.62 ± 4.95^b	22.86 ± 2.45	32.94 ± 2.45^a	23.66 ± 2.43
CD4⁺		19.65 ± 1.56^a	17.29 ± 4.47	12.00 ± 2.97^b	15.18 ± 1.27	22.07 ± 4.47^a	16.82 ± 1.70
CD8⁺		11.91 ± 2.97^a	8.84 ± 1.66	6.79 ± 1.96^b	7.93 ± 2.00	10.62 ± 2.29	7.06 ± 2.22
CD4⁺/CD25⁺		2.60 ± 0.65	1.92 ± 0.75	1.42 ± 0.48	1.49 ± 0.49	2.51 ± 0.76	1.24 ± 0.69
CD3⁺/CD49b⁺		2.11 ± 0.83	1.33 ± 0.06	1.39 ± 0.88	1.26 ± 0.21	1.34 ± 0.19	1.30 ± 0.47
CD49b⁺		4.86 ± 0.65	3.79 ± 0.73	4.25 ± 0.86	4.28 ± 0.40	3.91 ± 0.26	3.92 ± 0.72

Lowercase letters indicate the comparison of lymphocyte subsets within the same AIR mouse line (a > b, P < 0.05). Capital letters indicate a comparison of lymphocyte subsets between AIRmax and AIRmin (A > B, P < 0.05).