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. 2022 Mar 3;82(5):933–949.e9. doi: 10.1016/j.molcel.2022.01.008

Figure 2.

Figure 2

Super-resolution microscopy reveals nanoscopic BAK structures in apoptotic cells

(A) Gallery of BAK structures in apoptotic BAX/BAK DKO HCT116 cells transfected with mEGFP-BAK and labeled with an anti-GFP Alexa Fluor 647 nanobody. Scale bars, 100 nm.

(B) Relative distribution of the imaged BAK structure types over the total number of structures in all measured cells (n = 12).

(C and D) Quantification of the line and arc length and of the ring perimeter (C) and of the arc and ring radius (D) for all analyzed cells (n = 12).

(E) Quantification of the diameter of dots (blue) and outer diameter of rings (light blue) for all analyzed cells (n = 12).

(F) Exemplary images representing dots with less intensity in the center. Scale bars, 100 nm.

(G) Relative distribution of all collected line (minimum 84), arc (minimum 112), and ring (minimum 24) structures (cells n = 12) at different time points after first foci appearance. See also Figures S1 and S2.