REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Alpaca anti-GFP VHH sdAb AF647 (anti-GFP nanobody-AF647) 1:2000 dilution	Chromotec (home labeled)	Cat#gt-250
Rabbit polyclonal anti-BAX 1:1000 dilution	Cell Signaling Technology	Cat#2772; RRID: AB_10695870
Rabbit monoclonal anti-BAX (D2E11) 1:1000 dilution	Cell Signaling Technology	Cat#5023; RRID: AB_10557411
Mouse monoclonal anti-GFP 1:1000 dilution	ThermoFischer	Cat#MA5-15256; RRID: AB_10979281
Rabbit polyclonal anti-GAPDH 1:2500 dilution	abcam	Cat#ab9485; RRID: AB_307275
Goat polyclonal anti-Rabbit IgG-HRP 1:10000 dilution	Jackson Immuno Research	Cat#111-035-003; RRID: AB_2313567
Goat polyclonal anti-Mouse IgG-HRP 1:10000 dilution	Jackson Immuno Research	Cat# 115-035-003; RRID: AB_10015289
Camelid sdAb anti-GFP; Clones 1H1(FluoTag-Q anti-GFP Alexa Fluor 647)1:2000 dilution	NanoTag Biotechnologies	Cat#N0301
Goat polyclonal anti-Rabbit (AF633) 1:200 dilution	ThermoFisher	Cat#A-21070; RRID: AB_2535731
Rabbit monoclonal anti-Tom20 (D8T4N)1:100 dilution	Cell Signaling Technology	Cat#42406; RRID: AB_2687663
Mouse anti-Cytc 1:1000 dilution	BD Biosciences	Cat#556433; RRID: AB_396417
Mouse monoclonal anti-GAPDH (D4C6R)1:1000 dilution	Cell Signaling Technology	Cat#97166; RRID: AB_2756824
Rabbit anti-IMMT 1:1000 dilution	Proteintech	10179-AP; RRID: AB_2127193
Rabbit polyclonal anti-PARP 1:1000 dilution	Cell Signaling Technology	Cat#9542; RRID: AB_2160739
Rabbit monoclonal anti-pTBK1/NAK (Ser172) (D52C2)1:1000 dilution	Cell Signaling Technology	Cat#5483; RRID: AB_10693472
Rabbit monoclonal anti-TBK1/NAK (D1B4) 1:1000 dilution	Cell Signaling Technology	Cat#3504; RRID: AB_2255663
Rabbit monoclonal anti-STING (D2P2F) 1:1000 dilution	Cell Signaling Technology	Cat#13647; RRID: AB_2732796
PerCP/Cyanine5.5 Rat anti-mouse Ly-6C 1:200 dilution	Biolegend	Cat#128011; RRID: AB_1659242
Alexa Fluor 488 Rat anti-mouse/human CD44 1:800 dilution	Biolegend	Cat#103016; RRID: AB_493679
Alexa Fluor 647 Mouse anti-mouse NK-1.1 1:200 dilution	Biolegend	Cat#108719; RRID: AB_493186
Alexa Fluor 700 Rat anti-mouse CD4 1:100 dilution	Biolegend	Cat#100536; RRID: AB_493701
Super Bright 702 Rat monoclonal anti-CD45 (30-F11)1:200 dilution	ThermoFischer	Cat#67-0451-82; RRID: AB_2662424
Brilliant Violet 785 Rat anti-mouse CD62L 1:800 dilution	Biolegend	Cat#104440; RRID: AB_2629685
Brilliant Violet 650 Armenian Hamster anti-mouse CD80 1:400 dilution	Biolegend	Cat#104732; RRID: AB_2686972
Brilliant Violet 421 Rat anti-mouse/human CD11b 1:400 dilution	Biolegend	Cat#101251; RRID: AB_2562904
Brilliant Violet 510 Rat anti-mouse CD8a 1:200 dilution	Biolegend	Cat#100752; RRID: AB_2563057
Super Bright 600 Rat monoclonal anti-CD45R (B220) (RA3-6B2) 1:200 dilution	ThermoFischer	Cat#63-0452-82; RRID: AB_2637457
PE-Cy7 Hamster anti-mouse CD11c 1:400 dilution	BD Biosciences	Cat#561022; RRID: AB_647251
PE Rat anti-mouse Ly-6G 1:800 dilution	Biolegend	Cat#127607; RRID: AB_1186104
PE/Dazzle 594 Rat anti-mouse I-A/I-E 1:800 dilution	Biolegend	Cat#107647; RRID: AB_2565978
Rat anti-mouse CD16/CD32 1:50 dilution	BD Biosciences	Cat#553142; RRID: AB_394656
Bacterial and virus strains
BL21 (DE3) RIPL	Stratagene (available via Agilent)	Cat#230280
Chemicals, peptides, and recombinant proteins
Egg PC	OttoNordwald (Avanti)	Cat#840051C
Cardiolipin	Sigma	Cat#C0563
DiI stain	ThermoFischer	Cat#D3911
S63845	Hölzel	Cat#HY-100741
ABT-737	Hölzel	Cat#HY-50907
Q-VD-OPh	Hölzel	Cat#HY-12305g
Staurosporine	LC laboratories (USA)	Cat#S3900
GLUCOSE OXIDASE TYPE VII	Sigma	Cat#G2133-10KU
Cysteamine-Hydrochlorid (MEA)	Sigma	Cat#95294-1L
Catalase	Merck	Cat#219261-100KU
SNAP-cell 647SiR	NEB	Cat#S9102S
Janelia Fluor® 549 HaloTag® Ligand	Promega	Cat#GA1110
Live Cell Imaging Solution	ThermoFischer	Cat#A14291DJ
Pierce™ Streptavidin Magnetic Beads	ThermoFischer	Cat#88817
GFP Trap agarose beads	Chromotek	Cat#X336.1
XIRAN® (SMA2:1)	Polyscope	Cat#SZ30010
Critical commercial assays
Live/Dead fixable Near-IR Cell Stain Kit	ThermoFischer	Cat#L10119
Deposited data
Raw western blot and microscopy data	Mendeley	doi: 10.17632/2gwcgpvwx5.1
Experimental models: Cell lines
Human bone osteosarcoma cells: U2OS	Gift from S. Tait, Glasgow	https://doi.org/10.15252/embj.201899238
Human bone osteosarcoma cells: U2OS BAX -/- (U2OS BAX KO)	Gift from S. Tait, Glasgow	https://doi.org/10.15252/embj.201899238
Human bone osteosarcoma cells: U2OS BAK -/-(U2OS BAK KO)	Gift from S. Tait, Glasgow	https://doi.org/10.15252/embj.201899238
Human bone osteosarcoma cells: U2OS BAX -/-/BAK -/-(U2OS BAX/BAK DKO)	Gift from S. Tait, Glasgow	https://doi.org/10.15252/embj.201899238
Human bone osteosarcoma cells: U2OS NUP96-mEGFP	Available from CLS cell lines service GmbH	Cat#300174
Murine endothelial cells: SVEC 4–10	Gift from S. Tait, Glasgow	https://doi.org/10.15252/embj.201899238
Murine endothelial cells: SVEC 4–10 BAX -/-(SVEC BAX KO)	Gift from S. Tait, Glasgow	https://doi.org/10.15252/embj.201899238
Murine endothelial cells: SVEC 4–10 BAK -/-(SVEC BAK KO)	Gift from S. Tait, Glasgow	https://doi.org/10.15252/embj.201899238
Human colon cancer cells: HCT116 BAX -/-/BAK -/-(HCT116 BAX/BAK DKO)	Gift from K. Schulze-Osthoff, Tübingen	N/A
Spleenocytes extracted from female mice (C57BL/6N)	Available from The Jackson Laboratory	Cat#005304
Recombinant DNA
pcDNA3_Smac1-60_mCherry	Gift from S. Tait, Glasgow	https://doi.org/10.15252/embj.201899238
mTagBFP2-TOMM20-N-10	Addgene	Cat#55328
pEGFP-A206K_BAX_C1 (mEGFP-BAX)	This manuscript	N/A
pEGFP-A206K_BAX(S184V) C1 (mEGFP-BAX(S184V))	This manuscript	N/A
pEGFP-A206K_BAX(T182I) C1 (mEGFP-BAX(T182I))	This manuscript	N/A
pEGFP_A206K_BAK_C1 (mEGFP-BAK)	This manuscript	N/A
pTYB21_mBAK-L192K, F193S, Y196D, C154S	This manuscript	N/A
pTYB1_hBAX-S4C,C62S, C126S	Previous study	https://doi.org/10.1038/ncomms9042
pTYB1 Bid WT	Previous study	https://doi.org/10.1038/ncomms9042
pEGFP-Tfam-GFP	This manuscript	N/A
pCRY2(1-531)-mCherry-BAK	Addgene	Cat#117238
pcDNA-CIBN-mCherry-BAK	This manuscript	N/A
pEGFP-N1-4xmt-mTurquoise2	Addgene	Cat#98819
pAcGFP-C1-SnapF-BAX	This manuscript	N/A
pAcGFP-C1-Halo7-BAK	This manuscript	N/A
pEGFP-Flag-APEX2-BAX	This manuscript	N/A
pEGFP-Flag-APEX2-BAK	This manuscript	N/A
pAcGFP-C1-RA-BAX	This manuscript	N/A
pAcGFP-C1-GB-BAK	This manuscript	N/A
Software and algorithms
Stoichiometry Analysis Software (SAS)	https://doi.org/10.1021/acs.jpclett.1c03835	https://github.com/jdanial/SAS
GUVdetector software	https://doi.org/10.1093/bioinformatics/btu102	http://garcia-saez.cecad-labs.uni-koeln.de/GUV-software.864.0.html
ImageJ/FIJI	doi: 10.1038/nmeth.2019	https://imagej.net/software/fiji/
ThunderStorm	DOI: 10.1093/bioinformatics/btu202	https://code.google.com/p/thunder-storm/
Huygens Professional	Scientific Volume Imaging, Netherlands	http://svi.nl
Radial Profile Plot Plugin (ImageJ)	https://imagej.nih.gov/ij/plugins/radial-profile.html	N/A
GraphPad Prism 5.0	GraphPad	https://www.graphpad.com/scientific-software/prism/
Origin	OriginLab Corporation	https://www.originlab.com/
Python	https://www.python.org/	N/A