TABLE 3.
Evaluation of sensitivity of BACTEC broth method for detection of resistance to RMP and RPT in strains containing small proportions of resistant bacteria
| Strain and % resistant bacteria incorporated into inoculum | % Resistant bacteria actually found on agar plates containing drug at the following concn (μg/ml):
|
RMP MIC (μg/ml)
|
RPT MIC (μg/ml)
|
||||||
|---|---|---|---|---|---|---|---|---|---|
| 0.25 | 0.5 | 2.0 | 4.0 | 8.0 | Agar method | BACTEC method | Agar method | BACTEC method | |
| H37Rv | |||||||||
| 1 | 4 | 2 | 2 | 4 | 4 | >8.0 | 0.12 | ||
| 10 | 16 | 14 | 9 | 10 | 6 | >8.0 | 4.0 | ||
| 50 | 100 | 60 | 39 | 84 | 71 | >8.0 | >8.0 | ||
| 100 | 100 | 100 | 100 | 100 | 100 | >8.0 | >8.0 | ||
| Atencio | |||||||||
| 1 | 8 | 3 | 4 | 2 | 1 | >8.0 | 0.12 | ||
| 10 | 10 | 18 | 11 | 14 | 16 | >8.0 | 8.0 | ||
| 50 | 42 | 67 | 43 | 100 | 83 | >8.0 | >8.0 | ||
| 100 | 100 | 100 | 100 | 100 | 100 | >8.0 | >8.0 | ||
| Erdman | |||||||||
| 1 | 3 | 2 | 2 | 1 | 2 | >8.0 | 0.12 | ||
| 10 | 15 | 20 | 19 | 19 | 23 | >8.0 | 8.0 | ||
| 50 | 92 | 86 | 57 | 69 | 74 | >8.0 | >8.0 | ||
| 100 | 100 | 100 | 100 | 100 | 100 | >8.0 | >8.0 | ||
| H37Rv | |||||||||
| 1 | 4 | 3 | 3 | 2 | 4 | >8.0 | 0.06 | ||
| 10 | 10 | 10 | 10 | 17 | 12 | >8.0 | 4.0 | ||
| 50 | 63 | 55 | 39 | 43 | 45 | >8.0 | >8.0 | ||
| 100 | 100 | 100 | 100 | 100 | 100 | >8.0 | >8.0 | ||
| Atencio | |||||||||
| 1 | 3 | 3 | 5 | 4 | 2 | >8.0 | 0.06 | ||
| 10 | 27 | 30 | 30 | 22 | 27 | >8.0 | 8.0 | ||
| 50 | 93 | 100 | 99 | 85 | 100 | >8.0 | >8.0 | ||
| 100 | 100 | 100 | 100 | 100 | 100 | >8.0 | >8.0 | ||
| Erdman | |||||||||
| 1 | 3 | 1 | 0 | 0.7 | 0 | 2.0 | 0.06 | ||
| 10 | 19 | 19 | 9 | 15 | 16 | >8.0 | 8.0 | ||
| 50 | 83 | 84 | 96 | 78 | 70 | >8.0 | >8.0 | ||
| 100 | 100 | 100 | 100 | 100 | 100 | >8.0 | >8.0 | ||