Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Feb 22;13:805451. doi: 10.3389/fimmu.2022.805451

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Gao, Cao, Deng, Yang, Song, Ni, Xie, Fan, Ou, Huang, Lin, Liu, Li, Sun, Cheng, Wu, Xia, Deng, Mou and Chen

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

The temporal dynamics of mTEC diversity during thymus organogenesis. (A) UMAP visualization of 5,493 mTECs with 9 subclusters. (B) Percentages of 8 mTEC subtypes across the development from E13.5 to adult. (C) UMAP visualization of the expression of curated feature genes specific for 4 mTEC subtypes. (See also Figure S3A .) (D) Single-molecule RNA fluorescence in situ hybridization in fetal thymus slides with probes targeting Foxn1 and Ccl21a. (E–H) Immunofluorescence staining of 5-week thymus slides with antibodies to Krt5, Emp2 (E), Hnf1a (F), Ascl1 (G), Cdx1 (H), and Krt8 (C, cortex; M, medulla). (I) UMAP visualization of the expression of curated feature genes specific for 4 mTEC subtypes: Spink5⁺ cells, Bpifa1⁺ cells, tuft-like cells, and Ccl6⁺ cells. (See also Figure S4A ) (J) Immunofluorescence staining of 5-week thymus slides with antibodies to Krt5 (green), Ccl6 (red), and Krt8 (blue). All staining experiments were replicated at least 2 times, with 2 biological replicates for each experiment.