Table 3:

Performance indicators for detection of cervical intraepithelial neoplasia grade 2 or worse (CIN2+) or grade 3 or worse (CIN3+) using (1) cycle threshold values on the three channels detecting HPV 16, 18, 45, 31, 33, 35, 52, 58 (HPV test only) or (2) delta cycle threshold values for CDKN2A and MKi67 measured if the HPV test was positive for any high risk types (reflex testing) by HIV status.

	Area Under the Curve (95% confidence interval)	Sensitivity	Specificity	Positive Predictive Value	Negative Predictive Value	Screen Positive
CIN2+
1) HPV testing only: Cycle threshold values on channels detecting HPV 16, 18, 45, 31, 33, 35, 52, 58
Without HIV	0.9059 (0.8658 – 0.9460)	80.00	93.96	41.87	98.86	9.86
Living with HIV	0.8705 (0.8354 – 0.9056)	80.00	83.27	48.20	95.53	27.04
2) Reflex testing: CDKN2A and MKi67 if HPV-positive (any high risk)
Without HIV	0.9171 (0.8799 – 0.9563)	80.00	95.17	47.39	98.87	8.71
Living with HIV	0.8734 (0.8389 – 0.9079)	80.00	81.32	45.46	95.43	28.67
CIN3+
1) HPV testing only: Cycle threshold values on channels detecting HPV 16, 18, 45, 31, 33, 35, 52, 58
Without HIV	0.9235 (0.8836 – 0.9633)	80.00	93.77	31.38	99.25	6.71
Living with HIV	0.8701 (0.8304 – 0.9097)	80.00	85.36	34.49	97.79	20.39
2) Reflex testing: CDKN2A and MKi67 if HPV-positive (any high risk)
Without HIV	0.9398 (0.9019 – 0.9769)	80.00	94.96	36.10	99.26	5.56
Living with HIV	0.8756 (0.8368 – 0.9137)	80.00	81.07	28.94	97.68	24.30