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. 2021 Sep 29;29(3):527–539. doi: 10.1038/s41418-021-00872-2

Fig. 1. Identification of CNEACR in mice hearts with ischemia/reperfusion (I/R) injury.

Fig. 1

a and b The expression levels of circRNAs (selected from circRNA microarray data) in I/R injured mice hearts determined by qPCR (n = 5 independent experiments). c The representative agarose gel image showing the linear or back-splicing products amplified using convergent and divergent primers of CNEACR in mice hearts. d Sanger sequencing was performed to confirm head-to-tail back-spliced site in CNEACR (black arrow). e QPCR analysis showing the expression level of CNEACR in cardiomyocytes and fibroblasts isolated from mice hearts (n = 3 independent experiments). f The level of CNEACR in the cytoplasmic or nuclear fractions of isolated cardiomyocytes as determined by qPCR. U6 and GAPDH used as internal controls (n = 3 independent experiments). g Representative images of fluorescence in situ hybridization with junction-specific probes of CNEACR indicates its subcellular localization. Green represents CNEACR and blue labels nuclei. Scale bar, 20 μm. Data in graphs are presented as Mean ± s.d. P values were calculated using two-sided Student’s t test.