Table 1.
Iba1+-microglia predict episodic memory whereas IFNγ and CXCL1 predict spatial memory in J20.
| Predicting NOR episodic memory deficits | |||||
|---|---|---|---|---|---|
| Marker (independent variable) | Coefficient | Standard error | t value | p value | |
| Histology markers | Intercept | 0.71 | 0.30 | 2.35 | 0.037 |
| Hippocampus | Synaptophysin+ staining | 0.13 | 0.27 | 0.47 | 0.65 |
| Iba1+ microglia | −0.50 | 0.21 | −2.38 | 0.035 | |
| Aβ+ staining | −0.059 | 0.036 | −1.62 | 0.13 | |
| Biochemistry markers | Intercept | 0.68 | 0.39 | 1.73 | 0.10 |
| Hippocampus | Spine Density | −0.049 | 0.031 | −1.60 | 0.13 |
| TNFα | −0.85 | 0.99 | −0.86 | 0.40 | |
| IFNγ | 13.56 | 8.27 | 1.64 | 0.12 | |
| CXCL1 | 0.0082 | 0.0093 | 0.89 | 0.39 | |
| Total Aβ | −0.12 | 0.075 | −1.62 | 0.13 | |
| Predicting Barnes maze spatial memory deficits | |||||
|---|---|---|---|---|---|
| Marker (independent variable) | Coefficient | Standard error | t value | p value | |
| Histology markers | Intercept | 1.75 | 1.53 | 1.15 | 0.27 |
| Hippocampus | Synaptophysin+ staining | −1.02 | 1.33 | −0.77 | 0.45 |
| Iba1+ microglia | 0.53 | 0.54 | 0.97 | 0.35 | |
| Aβ+ staining | −0.15 | 0.18 | −0.84 | 0.42 | |
| Biochemistry markers | Intercept | 4.019 | 1.16 | 3.45 | 0.0039 |
| Hippocampus | Spine Density | −0.012 | 0.092 | −0.13 | 0.90 |
| TNFα | 2.038 | 3.00 | 0.68 | 0.51 | |
| IFNγ | −95.17 | 25.64 | −3.71 | 0.0023 | |
| CXCL1 | −0.091 | 0.028 | −3.23 | 0.0060 | |
| Total Aβ | 0.18 | 0.23 | 0.79 | 0.44 | |
Linear regression model results determining which hippocampal variable associates with NOR episodic memory (top) and Barnes maze spatial memory (bottom) deficits. Analyses were performed on WT, J20, J20/Nlrp1−/−, J20/Casp1−/−, and J20/Casp6−/− mice with complete data only. Markers separated into two separate models based on paraformaldehyde fixed or frozen brain tissue processing. Model one combines immunohistochemical markers synaptophysin staining, Iba1+ microglia, and Aβ staining in the hippocampus (n = 16). Model two combines Golgi–cox-stained spine density, pro-inflammatory TNFα, IFNγ, and CXCL1 (cytokines that previously showed significant changes in J20 mice), and total formic acid-soluble Aβ protein levels (n = 21). The large values for Iba1, Aβ staining, and total Aβ were re-scaled to bring coefficients into an acceptable range but does not affect t or p values.