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. 2022 Mar 7;12:3694. doi: 10.1038/s41598-022-07739-7

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© The Author(s) 2022, corrected publication 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Determination of mechanical properties of 10% w/v (10%), 15% w/v (15%), 20% w/v (20%) gelatin membranes. (a) Analysis of storage and loss modulus of each membrane under applied oscillatory compression. (b) Comparison of 20 µM Fluorescein sodium salt dye transport through each membrane assembled to 96-well plate format 3D-printed strip insert wells (10–20%: n = 4; PTFE: n = 7). Insert well aperture surface area for transport is 0.05 cm². (c) Determination of thickness changes of 15% (w/v) Gelatin membranes assembled to 48-well format (Ø: d = 8.8 mm; a = 4 mm); wet (n = 5), swollen (n = 5), and dry (n = 5). (d) Thicknesses measurement of 15% (w/v) gelatin membranes (n = 5) via fluorescence intensity analysis. Error bars indicate mean ± SD. Statistical significance: ns = P > 0.05, ****P < 0.0001.