Skip to main content
. 2021 Sep 20;29(3):504–513. doi: 10.1038/s41418-021-00870-4

Fig. 4. Identification of the Caspase 3 target site in Otulin.

Fig. 4

A HT1080-OT-KD cells expressing HA-Otulin were transfected with poly (I:C) (1 μg) for 24 h to activate RIPA and analyzed for Otulin cleavage using HA and Otulin antibody. MG132 treatment was given for 4 h before harvesting cells. B Multiple sequence alignments showing conservation of Caspase 3 site D31 in various organisms. C Otulin WT and Otulin D31A expressing OT-KD cells were infected with SeV (MOI 10) for 24 h followed by MG132 treatment of 4 h before harvesting the cells. Otulin cleavage was detected using Otulin antibody (mean ± SEM, n = 3; ns > 0.05; *P < 0.05; **P < 0.01, ***P < 0.001).