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. 2022 Mar 7;13(3):212. doi: 10.1038/s41419-022-04660-9

Fig. 1. Plasma-activated medium induces cancer cell death by regulating intracellular ROS.

Fig. 1

The plasma exposure conditions were 1.9 standard liter per minute (SLM), 7 W input power and three exposure times (60, 120, and 180 sec). A The cell viability and cytotoxicity were measured using MTT and LDH assay. B Intracellular ROS was detected at 24 h after PAM treatment. C PAM regulated mRNA expression levels of oxidative stress genes in H1299 and A549 cancer cells (exposure time: 180 sec). D N-acetylcysteine (NAC, 2 mM) recovered cell viability and suppressed intracellular ROS by PAM treatment. Cells were treated with PAM (180 sec) for 24 h. Data represent the mean ± SD from three independent experiments (*p < 0.05, **p < 0.01 and ***p < 0.001, as determined by t test).